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phospho creb 1 ser133  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology phospho creb 1 ser133
    Phospho Creb 1 Ser133, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 364 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phospho+creb+1/CREB-1/10__1096_slash_fj__202501468r-59-45-61
    Average 95 stars, based on 364 article reviews
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    Article Title: Metabotropic glutamate 2,3 receptor stimulation desensitizes agonist activation of G-protein signaling and alters transcription regulators in mesocorticolimbic brain regions.
    Article Snippet: Primary antibodies which recognize α, β, and δ CREB isoforms (CREB-1 (sc-377154), and phospho-CREB-1 (sc-101663)) were from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

    Article Title: Xinbao pill attenuated water retention by regulating the CaSR/AQP2 pathway in LAD-induced chronic heart failure rats
    Article Snippet: Antibodies against phospho-p38MAPK (#sc-166182), AQP2 (#sc-515770), CREB-1 (#sc-377154c), phospho-CREB-1 (#sc-81486c), CaSR (#sc-47741c) were purchased from Santa Cruz Biotechnology, Inc (California, USA).

    Article Title: Metabotropic glutamate 2,3 receptor stimulation desensitizes agonist activation of G-protein signaling and alters transcription regulators in mesocorticolimbic brain regions
    Article Snippet: Primary antibodies which recognize α, β, and δ CREB isoforms (CREB-1 (sc-377154), and phospho-CREB-1 (sc-101663)) were from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

    Article Title: GCN2 regulates the CCAAT enhancer binding protein beta and hepatic gluconeogenesis
    Article Snippet: Primary antibodies for phospho enol pyruvate carboxykinase (PEPCK), C/EBPβ, CREB-1, phospho-CREB-1 (Ser 129 ) (Santa Cruz Biotech, Santa Cruz, CA), eIF2α and eIF2α(pS52) (Invitrogen, Camarillo, CA), and α-tubulin (Sigma) were used.

    Article Title: Xinbao pill attenuated water retention by regulating the CaSR/AQP2 pathway in LAD-induced chronic heart failure rats
    Article Snippet: Antibodies against phospho-p38MAPK (#sc-166182), AQP2 (#sc-515770), CREB-1 (#sc-377154c), phospho-CREB-1 (#sc-81486c), CaSR (#sc-47741c) were purchased from Santa Cruz Biotechnology, Inc (California, USA).



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    phospho creb 1 ser133  (Santa Cruz Biotechnology)
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    (A) Schematic of LC-spinal cord projections (Allen Brain Atlas), indicating the LC injection site. (B, C) Fluoro-ruby was injected into the LC, with colocalization of Fluoro-ruby and TH/DBH observed in both LC and SDH. Scale bars = 50 μm (B), 20 μm (C). (D, F) Colocalization <t>of</t> <t>p-CREB/c-Fos</t> (green) with the neuronal marker NeuN (red) in SDH. Scale bar = 20 μm. (E) Quantitative analysis of fluorescence intensity in (D) (n = 3). (G) Quantitative of c-Fos-positive neurons in SDH (n = 3). (H) Western blot analysis of c-Fos and TH expression in spinal cord tissue. (I) Quantitative analysis of c-Fos and TH protein level (n = 3). * P < 0.05, ** P < 0.01 vs. sham group. Data were expressed as mean ± SD.
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    (A) Schematic of LC-spinal cord projections (Allen Brain Atlas), indicating the LC injection site. (B, C) Fluoro-ruby was injected into the LC, with colocalization of Fluoro-ruby and TH/DBH observed in both LC and SDH. Scale bars = 50 μm (B), 20 μm (C). (D, F) Colocalization <t>of</t> <t>p-CREB/c-Fos</t> (green) with the neuronal marker NeuN (red) in SDH. Scale bar = 20 μm. (E) Quantitative analysis of fluorescence intensity in (D) (n = 3). (G) Quantitative of c-Fos-positive neurons in SDH (n = 3). (H) Western blot analysis of c-Fos and TH expression in spinal cord tissue. (I) Quantitative analysis of c-Fos and TH protein level (n = 3). * P < 0.05, ** P < 0.01 vs. sham group. Data were expressed as mean ± SD.
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    (A) Schematic of LC-spinal cord projections (Allen Brain Atlas), indicating the LC injection site. (B, C) Fluoro-ruby was injected into the LC, with colocalization of Fluoro-ruby and TH/DBH observed in both LC and SDH. Scale bars = 50 μm (B), 20 μm (C). (D, F) Colocalization <t>of</t> <t>p-CREB/c-Fos</t> (green) with the neuronal marker NeuN (red) in SDH. Scale bar = 20 μm. (E) Quantitative analysis of fluorescence intensity in (D) (n = 3). (G) Quantitative of c-Fos-positive neurons in SDH (n = 3). (H) Western blot analysis of c-Fos and TH expression in spinal cord tissue. (I) Quantitative analysis of c-Fos and TH protein level (n = 3). * P < 0.05, ** P < 0.01 vs. sham group. Data were expressed as mean ± SD.
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    (A) Schematic of LC-spinal cord projections (Allen Brain Atlas), indicating the LC injection site. (B, C) Fluoro-ruby was injected into the LC, with colocalization of Fluoro-ruby and TH/DBH observed in both LC and SDH. Scale bars = 50 μm (B), 20 μm (C). (D, F) Colocalization <t>of</t> <t>p-CREB/c-Fos</t> (green) with the neuronal marker NeuN (red) in SDH. Scale bar = 20 μm. (E) Quantitative analysis of fluorescence intensity in (D) (n = 3). (G) Quantitative of c-Fos-positive neurons in SDH (n = 3). (H) Western blot analysis of c-Fos and TH expression in spinal cord tissue. (I) Quantitative analysis of c-Fos and TH protein level (n = 3). * P < 0.05, ** P < 0.01 vs. sham group. Data were expressed as mean ± SD.
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    (A) Schematic of LC-spinal cord projections (Allen Brain Atlas), indicating the LC injection site. (B, C) Fluoro-ruby was injected into the LC, with colocalization of Fluoro-ruby and TH/DBH observed in both LC and SDH. Scale bars = 50 μm (B), 20 μm (C). (D, F) Colocalization <t>of</t> <t>p-CREB/c-Fos</t> (green) with the neuronal marker NeuN (red) in SDH. Scale bar = 20 μm. (E) Quantitative analysis of fluorescence intensity in (D) (n = 3). (G) Quantitative of c-Fos-positive neurons in SDH (n = 3). (H) Western blot analysis of c-Fos and TH expression in spinal cord tissue. (I) Quantitative analysis of c-Fos and TH protein level (n = 3). * P < 0.05, ** P < 0.01 vs. sham group. Data were expressed as mean ± SD.
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    (A) Schematic of LC-spinal cord projections (Allen Brain Atlas), indicating the LC injection site. (B, C) Fluoro-ruby was injected into the LC, with colocalization of Fluoro-ruby and TH/DBH observed in both LC and SDH. Scale bars = 50 μm (B), 20 μm (C). (D, F) Colocalization <t>of</t> <t>p-CREB/c-Fos</t> (green) with the neuronal marker NeuN (red) in SDH. Scale bar = 20 μm. (E) Quantitative analysis of fluorescence intensity in (D) (n = 3). (G) Quantitative of c-Fos-positive neurons in SDH (n = 3). (H) Western blot analysis of c-Fos and TH expression in spinal cord tissue. (I) Quantitative analysis of c-Fos and TH protein level (n = 3). * P < 0.05, ** P < 0.01 vs. sham group. Data were expressed as mean ± SD.
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    Image Search Results


    (A) Schematic of LC-spinal cord projections (Allen Brain Atlas), indicating the LC injection site. (B, C) Fluoro-ruby was injected into the LC, with colocalization of Fluoro-ruby and TH/DBH observed in both LC and SDH. Scale bars = 50 μm (B), 20 μm (C). (D, F) Colocalization of p-CREB/c-Fos (green) with the neuronal marker NeuN (red) in SDH. Scale bar = 20 μm. (E) Quantitative analysis of fluorescence intensity in (D) (n = 3). (G) Quantitative of c-Fos-positive neurons in SDH (n = 3). (H) Western blot analysis of c-Fos and TH expression in spinal cord tissue. (I) Quantitative analysis of c-Fos and TH protein level (n = 3). * P < 0.05, ** P < 0.01 vs. sham group. Data were expressed as mean ± SD.

    Journal: PLOS One

    Article Title: Alteration of BDNF and noradrenergic markers in locus coeruleus in a mouse model of cancer-induced bone pain

    doi: 10.1371/journal.pone.0330207

    Figure Lengend Snippet: (A) Schematic of LC-spinal cord projections (Allen Brain Atlas), indicating the LC injection site. (B, C) Fluoro-ruby was injected into the LC, with colocalization of Fluoro-ruby and TH/DBH observed in both LC and SDH. Scale bars = 50 μm (B), 20 μm (C). (D, F) Colocalization of p-CREB/c-Fos (green) with the neuronal marker NeuN (red) in SDH. Scale bar = 20 μm. (E) Quantitative analysis of fluorescence intensity in (D) (n = 3). (G) Quantitative of c-Fos-positive neurons in SDH (n = 3). (H) Western blot analysis of c-Fos and TH expression in spinal cord tissue. (I) Quantitative analysis of c-Fos and TH protein level (n = 3). * P < 0.05, ** P < 0.01 vs. sham group. Data were expressed as mean ± SD.

    Article Snippet: Endogenous peroxidase activity was blocked by incubating sections in 3% hydrogen peroxide for 10 min. Primary antibody incubation was performed at 4°C for 24 h using the following antibodies: BDNF (DF6387, rabbit, 1:100; Affinity), phospho-CREB (AF3189, rabbit, 1:100; Affinity), c-Fos (AF5354, rabbit, 1:100; Affinity), tyrosine hydroxylase (A25683, rabbit, 1:200; ABclonal), DBH (10777–1-AP, rabbit, 1:100; Proteintech), adrenergic receptor α-2A (DF3076, rabbit, 1:100; Affinity), NeuN (66836–1-Ig, mouse, 1:100; Proteintech), GABRB2 (A1876, rabbit, 1:100; ABclonal), PENK (A6302, rabbit, 1:100; ABclonal), and tyrosine hydroxylase (#45648, mouse, 1:200; Cell Signaling Technology).

    Techniques: Injection, Marker, Fluorescence, Western Blot, Expressing